Expression profile of transcripts encoding cell wall remodelling proteins in tomato fruit cv. Micro-Tom subjected to 158C storage

MÜLLER, GABRIELA LETICIA; BUDDE, CLAUDIO OLAF; LAUXMANN, MARTÍN ALEXANDER; TRIASSI, AGUSTINA; ANDREO, CARLOS SANTIAGO; DRINCOVICH, MARÍA FABIANA; LARA, MARÍA VALERIA

FUNCTIONAL PLANT BIOLOGY

CSIRO PUBLISHING

Lugar: Collingwood; Año: 2013 vol. 40 p. 449 - 458

1445-4408

To extend fruit market life, tomatoes are harvested before red ripe and kept at temperatures below optimum(20C). In this work, Micro-Tom tomatoes stored at 20C (normal ripening) were compared with those stored at 15Cor 4C(chilling injury inducer) for 7 days. In contrast to 4C, storage at 15C delayed ripening with the benefit of not enhancingoxidative metabolism and of enabling ripening upon being transferred to 20C. The transcriptional expression profile ofenzymes related to cell wall metabolism was compared at the three temperatures. Although endo-b-1,4-glucanase (Cel1),which is associated with fruit decay, was largely increased after removal from 4Cstorage, its expression was not modified infruits stored at 15C. Enhanced transcriptional expression of xyloglucan endotransgylcosylase/hydrolases (XTHs)XTH1, ?2,?10 and ?11, and of two b-xylosidases (Xyl1?2) was detected in fruits stored at 15Cwith respect to those at 20C. Following2 days at 20C, these transcripts remained higher in fruits stored at 15Cand XHT3 and ?9 also increased. Ethylene evolutionwas similar in fruits kept at 15C and 20C; thus, the changes in the transcript profile and fruit properties between thesetreatments may be under the control of factors other than ethylene.