CEFOBI 05405
CENTRO DE ESTUDIOS FOTOSINTETICOS Y BIOQUIMICOS
Unidad Ejecutora - UE
artículos
Título:
¨Differential fumarate regulation of Arabidopsis NAD+-malic enzymes 1 and -2 produces an opposite activity modulation¨
Autor/es:
TRONCONI, M.; GERRARD WHEELER M; DRINCOVICH MF,; ANDREO, C.S.
Revista:
BIOCHIMIE
Editorial:
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Referencias:
Lugar: Paris; Año: 2013 vol. 94 p. 1421 - 1430
ISSN:
0300-9084
Resumen:
Arabidopsis mitochondria contain two NAD+-malic enzymes, NAD-ME1 and NAD-ME2. These proteinshave similar affinity for their substrates but display opposite regulation by fumarate, which stronglystimulates NAD-ME1 but inhibits NAD-ME2 activity. Here, the interaction of NAD-ME1 and -2 withfumarate was investigated by kinetic approaches, urea denaturation assays and intrinsic fluorescencequenching, in the absence and presence of NAD+. Fumarate inhibited NAD-ME2 at
