Analysis of UV-B regulated miRNAs and their targets in maize leaves

PAULA CASATI

Plant Signaling and Behaviour

Landes Bioscience

Año: 2013 vol. 8 p. 10 - 10

1559-2324

UV-B radiation elicits physiological and developmental changes in plants. In this work, to characterize the mechanismsof gene regulation by UV-B radiation, the global miRNA expression profiles in juvenile maize leaves were compared undercontrol conditions in the absence of UV-B and after an 8 h-UV-B treatment similar to ambient UV-B levels using small RNAmicroarrays. Seventeen miRNA s were identified that were differentially expressed in response to UV-B. In parallel, theexpression of putative mRNA targets was examined in mRNA microarrays using the same RNA samples used for the smallRNA analysis. In general, there is a strong negative correlation to the miRNA expression. Thus, post-transcriptional regulationis an important step in gene expression by UV-B radiation in maize.