CEFOBI   05405
CENTRO DE ESTUDIOS FOTOSINTETICOS Y BIOQUIMICOS
Unidad Ejecutora - UE
artículos
Título:
The role of histidine in a copper-specific metallothionein
Autor/es:
PEREZ-RAFAEL SILVIA; PAGANI AYELEN; PALACIOS OSCAR; DALLINGER REINHARD; CAPDEVILA MERCE; ATRIAN SILVIA
Revista:
ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE
Editorial:
WILEY-V C H VERLAG GMBH
Referencias:
Lugar: Weinheim; Año: 2013 p. 1356 - 1360
ISSN:
0044-2313
Resumen:
Metallothioneins achieve metal binding specificity by modulation of their amino acid sequences through evolution. Non-coordinating residues seem to play a key role in this function, and among them histidine may be of particular importance. Here we report how this residue regulates CuI binding to a highly copper specific isoform, the CuMT of the snail Helix pomatia, by analysis of the recombinant complexes yielded by a constructed mutant where this residue has been changed to an alanine.