CEFOBI   05405
CENTRO DE ESTUDIOS FOTOSINTETICOS Y BIOQUIMICOS
Unidad Ejecutora - UE
artículos
Título:
Evolution and expression of tandem duplicated maize flavonol synthase genes
Autor/es:
MA LORENA FALCONE FERREYRA; MA. ISABEL CASAS; JULIA I. QÜESTA; L. HERRERA; S. DEBLASIO; J. WANG; D. JACKSON; E. GROTEWOLD; PAULA CASATI
Revista:
FRONTIERS IN BIOSCIENCE-LANDMARK
Editorial:
FRONTIERS IN BIOSCIENCE INC
Referencias:
Lugar: Albertson, NY, USA; Año: 2012 p. 101 - 101
ISSN:
1093-9946
Resumen:
Flavonoidsarespecializedcompoundswidelydistributedandwithdiversefunctionsthroughouttheplantkingdomandwithseveralbenefitsforhumahealth.Inparticular,flavonols,synthesizedbyflavonolsynthase(FLS),protectplantsagainstUV-Bradiationandareessentialformalefertilityinmaizeandotherplants.WehaverecentlycharacterizedaUV-BinducibleZmFLS1,correspondingtothefirsttobedescribedinmonocotplants.Inter-estingly,thenewassemblyoftheB73maizegenomerevealedthepresenceofasecondputativeFLSgene(ZmFLS2),withveryhighidentitywithZmFLS1.ZmFLSsexpressionwasanalyzedindifferentmaizetissues,andbycombiningelectrophoreticmobilityshiftassaysandtransientexpressionexperiments,weshowthatbothgenesaredirecttargetsofanthocyanin(C1/PL1+R/B)and3-deoxyflavonoid(P1)transcriptionalregulators.ZmFLSexpressionanalysesshowhigherlevelsofbothtranscriptsinhighaltitudelandracesthaninbredlines,andbothgenesareregulatedbyUV-Bradiationinalllinesanalyzed.Moreover,thehighsequenceconservationoftheZmFLSpromotersbetweenmaizelinessuggeststhatthedifferencesobservedinZmFLSexpressionareduetoallelcvariationsinthetranscriptionfactorsthatregulatetheiractivities.Finally,wegeneratedpFLS1::FLS1-RFPtransgenicplantsandanalyzedZmFLS1expressionindifferentmaizetissues;wefoundthatthisenzymeislocalizedintheERandtheperinuclearregion.