CEFOBI   05405
CENTRO DE ESTUDIOS FOTOSINTETICOS Y BIOQUIMICOS
Unidad Ejecutora - UE
artículos
Título:
Transcriptomic, proteomic and metabolomic analysis of UV-B signaling in maize
Autor/es:
CASATI, P; CAMPI M.; DARREN MORROW; FERNANDES J; WALBOT V.
Revista:
BMC GENOMICS
Editorial:
BIOMED CENTRAL LTD
Referencias:
Año: 2011 vol. 12 p. 321 - 321
ISSN:
1471-2164
Resumen:
Abstract
Background: Under normal solar fluence, UV-B damages macromolecules, but it also elicits physiological
acclimation and developmental changes in plants. Excess UV-B decreases crop yield. Using a treatment twice solar
fluence, we focus on discovering signals produced in UV-B-irradiated maize leaves that translate to systemic
changes in shielded leaves and immature ears.
Under normal solar fluence, UV-B damages macromolecules, but it also elicits physiological
acclimation and developmental changes in plants. Excess UV-B decreases crop yield. Using a treatment twice solar
fluence, we focus on discovering signals produced in UV-B-irradiated maize leaves that translate to systemic
changes in shielded leaves and immature ears.
Results: Using transcriptome and proteomic profiling, we tracked the kinetics of transcript and protein alterations
in exposed and shielded organs over 6 h. In parallel, metabolic profiling identified candidate signaling molecules
based on rapid increase in irradiated leaves and increased levels in shielded organs; pathways associated with the
synthesis, sequestration, or degradation of some of these potential signal molecules were UV-B-responsive.
Exposure of just the top leaf substantially alters the transcriptomes of both irradiated and shielded organs, with
greater changes as additional leaves are irradiated. Some phenylpropanoid pathway genes are expressed only in
irradiated leaves, reflected in accumulation of pathway sunscreen molecules. Most protein changes detected occur
quickly: approximately 92% of the proteins in leaves and 73% in immature ears changed after 4 h UV-B were
altered by a 1 h UV-B treatment.
Using transcriptome and proteomic profiling, we tracked the kinetics of transcript and protein alterations
in exposed and shielded organs over 6 h. In parallel, metabolic profiling identified candidate signaling molecules
based on rapid increase in irradiated leaves and increased levels in shielded organs; pathways associated with the
synthesis, sequestration, or degradation of some of these potential signal molecules were UV-B-responsive.
Exposure of just the top leaf substantially alters the transcriptomes of both irradiated and shielded organs, with
greater changes as additional leaves are irradiated. Some phenylpropanoid pathway genes are expressed only in
irradiated leaves, reflected in accumulation of pathway sunscreen molecules. Most protein changes detected occur
quickly: approximately 92% of the proteins in leaves and 73% in immature ears changed after 4 h UV-B were
altered by a 1 h UV-B treatment.
Conclusions: There were significant transcriptome, proteomic, and metabolomic changes under all conditions
studied in both shielded and irradiated organs. A dramatic decrease in transcript diversity in irradiated and
shielded leaves occurs between 0 h and 1 h, demonstrating the susceptibility of plants to short term UV-B spikes
as during ozone depletion. Immature maize ears are highly responsive to canopy leaf exposure to UV-B.
There were significant transcriptome, proteomic, and metabolomic changes under all conditions
studied in both shielded and irradiated organs. A dramatic decrease in transcript diversity in irradiated and
shielded leaves occurs between 0 h and 1 h, demonstrating the susceptibility of plants to short term UV-B spikes
as during ozone depletion. Immature maize ears are highly responsive to canopy leaf exposure to UV-B.