Multilobular Nanogels Design And Its Application In Transdermal Vaccination

GABRIEL CABRERA; ROQUE MINARI; GIULIANA LUPI; VERÓNICA GONZALEZ; ANA SONZOGNI; IVAN MARCIPAR

Simposio; XIII Simposio Argentino de Polímeros (SAP); 2019

Nanogels (NGs) are hydrogel nanoparticles based oncrosslinked hydrophilic polymer networks. NGs show a greatpotential in chemotherapy, diagnosis, organ targeting anddelivery of bioactive substances. Numerous synthesisstrategies were implemented to expand NGs properties focusin several applications, adding different functionalities,inorganic compounds and with natural polymers incorporation.However, the synthesis of biphasic polymer-polymer NGs hasnot been exploited yet. In a previous publication (Sonzogni etal. 2018a), we proposed the synthesis of bifunctional NGsbased on poly(N-vinylcaprolactam), PVCL, a thermo-responsivepolymer, and a copolymer rich on butyl acrylate (BA), whichpromotes particle coalescence (i.e., film formation). Thissystem offers the opportunity of combining both the highloading capacity of NGs and the delivery capability from a film,which could be previously obtained or directly formed onto thesurface where the release is required (i.e., in situ formed). Inthis scenario, the performance of this bifunctional NGs astopical delivery system of protein was compared with PVCLhydro and nanogels, and they showed a great potential as aplatform for dermal protein delivery (Sonzogni et al. 2018b).In this presentation we study the morphology and the processinvolved in the formation of this bifunctional NGs by asemibatch emulsion polymerization process. From thisinvestigation, a mechanism for the formation of thisbifunctional NGs was proposed. Also, the application ofbifunctional NGs as a transdermal vaccination platform,including in vivo results, is also included