A Hybrid Nanostructured Patch for Transdermal Protein Delivery

GONZALEZ, VERÓNICA D. G.; GEDTRICH, SARAH; SONZOGNI, ANA S.; CALDERÓN, MARCELO; KAR, MRITYUNJOY; MINARI, ROQUE J.; GUGLIOTTA, LUIS M.; YEALLAND, GUY

Simposio; Simposio Argentino de Polímeros (SAP2017); 2017

Topical delivery routes have a lot of advantages over other types of therapeutic agent delivery modalities such oral, intravenous, intramuscular, etc. Some positive features of this application route are the avoidance of first pass metabolism, of gastrointestinal incompatibility, drug level fluctuation, while minimizing undesirable side effect and providing a self-administration suitability [1]. Different designs of topical delivery systems were developed in order to improve their application, such as the cases of hydrogels in macro- and nano-scales (nanogels) [2][3].This work presents a novel platform for topical protein delivery. This platform consists in a nanostructured patch obtained from dispersed hybrid nanogels with film forming ability and thermosensitive properties. A semibatch strategy was designed to obtain nanogels based on poly(N-vinylcaprolactam), poly(butyl acrylate) and poly(acrylic acid). The poly(N-vinylcaprolactam) give them thermosensitive properties, and the other polymers are responsible of particles coalescence and skin adhesion. The performance of this patch is here compared with that of poly(N-vinylcaprolactam) macrogels and nanogels. Transition temperature, cytotoxicity, in vitro ovalbumin (as model protein) release-profile and ovalbumin skin penetration were evaluated and compared amongst the three systems. All the formulations resulted thermosensitive, non cytotoxic and able to load and release ovalbumin. The use of the nanostructured patch in topical delivery studies showed that skin penetration of ovalbumin was higher than that obtained with pure ovalbumin, and with nanogels and macrogels as supports. This result indicates that the proposed system could be used as platform for topical protein delivery.