INTEC   05402
INSTITUTO DE DESARROLLO TECNOLOGICO PARA LA INDUSTRIA QUIMICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Factors affecting the entrapment of florfenicol into polymeric nanoparticles
Autor/es:
LUDMILA TURINO; DIANA ESTENOZ; LUCIANO N. MENGATTO; FEDERICO KARP; JULIO A. LUNA
Lugar:
Rosario
Reunión:
Congreso; 4ta Reunión Internacional de Ciencias Farmacéuticas; 2016
Resumen:
Nanoparticles have been extensively investigated as drug delivery systems because of their especial features: release kinetics, cellular uptake, possibility of passive or active targeting, and parenteral administration. Poly(lactide-co-glycolide) (PLGA) has emerged as the most widely used and studied polymer for pharmaceutical applications due to its biodegradability and biocompatibility. The kinetic of drug release can be manipulated by varying it molecular weight, crystallinity, hydrophobicity and the end-group of the polymeric chains. In the present work, florfenicol (a broad spectrum antibiotic) was used as active ingredient. The aim was to study the influence of different factors in florfenicol/PLGA nanoparticles synthesis, using an oil-in-water emulsion?solvent evaporation technique. Experimental designs and statistical analysis were used for this purpose. First, a solution of PLGA in ethyl acetate containing florfenicol was mixed with a PVA aqueous solution. This mixture was homogenized using a probe sonicator during 2,5 minutes to produce the o/w emulsion. Finally, the organic solvent was evaporated during 1 hour using a rotative evaporator under partial vacuum. The factors studied were: PLGA concentration (0,5 ? 1 % w/v), PVA concentration (1 ? 2 % w/v) and oil-in-water proportion (1:3 ? 1:6). Analyzed responses were: mean size of particles and drug entrapment efficiency (EE). A complete factorial experimental design was used to study the system. The model used to explain the effects of the factors at the levels proposed was significant with a p-value less than 0,5. In the case of particles size, no factor affected significantly the response at the assayed levels. Particles between 150-240 nm were obtained. The significant factor that affected EE was the o/w proportion. According to this result, the EE can be enhanced reducing the o/w proportion. However, more levels of each factor should be investigated for a more precisely optimization.