Development of a Latex Agglutination Test for Acute Toxoplasmosis

PERETTI, LEANDRO E.; GONZALEZ, VERÓNICA D.G.; COSTA, JUAN G.; MARCIPAR, IVAN S.; GUGLIOTTA, LUIS M.

Porto de Galinhas, PE

Simposio; XIV Simposio Latinoamericano de Polímeros / XII Congreso Iberoamericano de Polímeros; 2014

Asociación Brasilera de Polímeros

The acute phase recombinant protein of Toxoplasma gondii P35Ag was expressed and purified; and homogenate of the parasite was obtained from infected mouse. This antigens were used to produce latex-protein complexes (LPC) through physical adsorption (PA) and chemical coupling (CC) at pH 6 onto latex particles of different sizes and charge densities, with the aim of producing immunoagglutination reagents able to detect Toxoplasmosis recently acquired. On sensitization experiments, P35Ag had better eficiency than homogenate, this would be because it is a complex mixture, which includes a large number of proteins of different molecular mass, isoelectric points and hydrophobicity. After the LPC characterization, they were employed in immunoagglutination assays. The agglutination reaction was followed by measuring the changes in the optical absorbance by turbidimetry. Experiments against a serum panel indicates that the IA test based on the latex-P35Ag complex could be used as a screening method for detecting acute Toxoplasmosis.