INTEC   05402
INSTITUTO DE DESARROLLO TECNOLOGICO PARA LA INDUSTRIA QUIMICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Hydration of peptides from the modeling of their electrophoretic mobility data
Autor/es:
MARÍA V. PIAGGIO; MARTA B. PEIROTTI; JULIO A. DEIBER
Lugar:
Buenos Aires
Reunión:
Simposio; 18 th Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical and Industrial Applications of Capillary Electrophoresis and Microchip Technology; 2012
Institución organizadora:
AES- Electrophoresis Society, USA
Resumen:
Peptides have associated a number of water molecules in a structural organization different from that expected in the bulk aqueous phase. The determination of this amount of water, usually designated hydration, is rather difficult, and its quantification may depend on the type of experimental technique used for this purpose [1]. In this work we study several peptides having a number of amino acid residues comprised in the range 5 to 42, by fitting their experimental electrophoretic mobility value [2] through basic models proposed in [3] and citations therein. Thus, the range of peptide hydration values is estimated by using electrically charged aspherical and spherical particles with occluded water in order to model electrokinetic and hydrodynamic phenomena. In general one finds that the minimum hydration is controlled by the peptide electrostatic state. Higher hydration levels may be obtained through a change of peptide shape by increasing particle sphericity. The same effect is obtained when certain solvent permeation through the particle is allowed, as it occurs by using spherical porous particles [4] and spherical soft particles [5]. The interplay between the topology defined by the chain amino acid sequence and its associated water molecules (hydration) seems to be relevant in the dynamic response of peptides in living systems. Therefore a discussion of typical hydrations values in relations to peptide physicochemical properties are presented. Subjects for future research are highlighted.   Acknowledgments: Authors wish to thank the financial aid received from Universidad Nacional del Litoral, Santa Fe, Argentina (CAI+D 2009) and CONICET (PIP 112-200801-01106).   References: [1] Piaggio MV, Peirotti MB, Deiber JA. Electrophoresis 2006, 27, 4631-4647. [2] Janini GM, Metral CJ, Isaaq HJ, Muschik GM. J. Chromatogr. A 1999, 848, 417-433. [3] Piaggio MV, Peirotti MB, Deiber JA. J. Sep. Sci. 2010, 33, 2423-2429. [4] Hermans JJ, Fujita H. Koninkl Ned. Akad. Wetenschap. Proc. Ser. B 1955, 58, 182-187. [5] Ohshima H. Electrophoresis 2006, 27, 526-533.