Influence of Hybrid-Chitosan Matrix Composition in the Covalent Multipoint Immobilization of Chymotrypsin

ADRIANO, WELLINGTON; MAMMARELLA, ENRIQUE; GIORDANO, RAQUEL

BIOMACROMOLECULES

ACS Publications

Lugar: Columbus, USA; Año: 2006

1525-7797

In this work, it was studied the development of chymotrypsin immobilization protocol into chitosan hybrids with different polymers and using cells on the production of derivatives by multipoint covalent attachment, as well as the activation influence on the obtained derivatives when compared to the free enzyme. The derivatives were analyzed as immobilization yield, coupling yield and stabilization factor at 55°C. The results showed that the best support activated with glutaraldehyde was chitosan 2.5% - alginate 2.5% - Bacillus megaterium 5%, being chitosan 2.5% - carrageenan 2.5% - Saccharomices cerevisiae 5% the best support when activated with glycidol and epichlorohydrin. However, chitosan 2.5% - carrageenan 2.5% - Saccharomices cerevisiae 5% activated with epichlorohydrin was 1424-fold more stable than free enzyme with maximum theoretical immobilized enzyme loading of approximately 40 mg enzyme.g-1 of support. The results were shown quite promising for projection of enzymatic hydrolysis of proteins at high temperatures.