INTEC   05402
INSTITUTO DE DESARROLLO TECNOLOGICO PARA LA INDUSTRIA QUIMICA
Unidad Ejecutora - UE
artículos
Título:
Immunodiagnosis of Chagas Disease: Synthesis of three Latex-Protein Complexes Containing different Antigens of Trypanosoma cruzi
Autor/es:
VERÓNICA D.G. GONZALEZ; VALERIA S. GARCIA; JORGE R. VEGA; IVAN S. MARCIPAR; GREGORIO R. MEIRA; LUIS M. GUGLIOTTA
Revista:
COLLOIDS AND SURFACES B-BIOINTERFACES
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2010 vol. 77 p. 12 - 17
ISSN:
0927-7765
Resumen:
This article describes the physical adsorption and the chemical coupling of 3 antigenic proteins of Trypanosoma cruzi onto polystyrene (PS) based latexes to be used as novel immunodiagnosis reagents for detecting the Chagas disease. The coupled proteins were a homogenate of T. cruzi, or a recombinant protein (either Ag36 or CP1). With the homogenate, between 30 and 60% of the total-linked protein was chemically coupled, showing a small dependence with the pH. For Ag36 and CP1, around 90% of the totallinked protein was chemically coupled, with a maximum coupling at pH 5 (i.e., close to the isoelectric point). The chemical coupling of CP1 was less affected by the pH than the coupling of Ag36.