INTEC   05402
INSTITUTO DE DESARROLLO TECNOLOGICO PARA LA INDUSTRIA QUIMICA
Unidad Ejecutora - UE
artículos
Título:
Immunodiagnosis of Chagas Disease: Synthesis of three Latex-Protein Complexes Containing different Antigens of Trypanosoma cruzi
Autor/es:
GONZALEZ, V. D. G.; GARCIA, V. S.; VEGA, J. R.; MARCIPAR, I. S.; MEIRA, G. R.; GUGLIOTTA, L. M.
Revista:
COLLOIDS AND SURFACES B-BIOINTERFACES
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2010 vol. 77 p. 12 - 17
ISSN:
0927-7765
Resumen:
This article describes the physical and chemical coupling of 3 antigenic proteins of Trypanosoma cruzi onto polystyrene (PS) based latexes; to be used as novel immunodiagnosis reagents for detecting the Chagas Disease. The coupled proteins were a homogenate of T. cruzi, or the recombinant proteins: Ag36 or CP1. With the homogenate, between 30 and 60% of the total linked protein was chemically-coupled; showing a small dependence with the pH. For Ag36 and CP1, around 90% of the total linked protein was chemically-coupled, with the maximum coupling at pH = 5 (i.e., close to the isoelectric point). The chemical coupling of CP1 was less affected by the pH than the coupling of Ag36.