INGAR   05399
INSTITUTO DE DESARROLLO Y DISEÑO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Co-production of dual bioinsecticide in a stirred tank bioreactor
Autor/es:
MICHELOUD, G.; GIORIA, V.; BECCARIA, A.; PÉREZ, G.; CLAUS, J.
Lugar:
Colonia-Uruguay
Reunión:
Taller; Segundo Taller Uruguayo de Agentes Microbianos de Control Biológico; 2008
Institución organizadora:
Instituto Nacional de Investigación Agropecuaria-Uruguay
Resumen:
The demand for environmentally friendly control methods and increasing problem of resistance to synthetic insecticides has led to the increased use of biological insecticides. The co-production of bioinsecticides is an interested proposes to improve the insecticide power besides to decrease the possibility of resistance cases. In this work, we evaluated the feasibility to produce a dual bioinsecticide composed by polyhedral inclusion bodies (PIBs) of Anticarsia gemmatalis multicapsid nucleopolyhedrovirus (AgMNPV) and toxin protein of Bacillus thuringiensis var. kurstaki (Btk). This dual bioinsecticide was produced in two phases in a batch process employing a 2 liter stirred reactor. The first phase was the viral infection and propagation on serum-free suspension culture of the saUFL-Ag-286 cell line. The parameters of infection were previously optimized in an experimental design. The second phase was the inoculation of the first bioinsecticide with Btk spores and cultured until the sporulation and production of toxin proteins. PIBs and spores concentration were determined using a Neubauer haemocytometer. The PIBs productivity obtained in the first period was 2,2x106 PIBs.mL-1.h-1 whereas the cellular specific yield of PIBs was 401 PIB/cell. The spores’ concentration at harvest time resulted 1,1x109 spores.mL-1. By means of bioassays was determined that the development of Btk on the first infected culture did not modify significantly the LD50 (50% lethal concentration) used when only PIBs were bioassayed. The possibility to reach good yields of PIBs and spores in only one batch process and the demonstration that Btk development is naive on PIBs produced in the first phase is an important advance towards the development of a feasible process to obtain an effective dual bioinsecticide in a low cost process.