A kinetic study on enzymatic hydrolysis of pretreated sugarcane bagasse

ALBERNAS CARVAJAL, YAILET; MESA, LEYANIS; CORSANO, GABRIELA; GONZALEZ SUAREZ, ERENIO; SANTOS, RONALDO; ROMERO, INMACULADA

Hamburgo

Congreso; 22nd European Biomass Conference and Exhibition; 2014

The enzymatic hydrolysis of pretreated bagasse as a cellulosic material was examined in a stirred flask reactor to establish a simplified kinetic model able to describe this complex process. A 23 experimental design was applied, using temperature, enzyme charge and solids concentration as factors and the Total Sugar production (ART) as a response. To enhance the saccharification of a pretreated bagasse with cellulase, the reaction was carried out employing Novozyme CellicRCTec2 and â-glucosidase code NS50010 cellulolitic enzymes. The kinetic model was proposed by regarding the reaction system as simplified pseudo-homogeneous Michaelis-Menten mechanism, in which sugars would be produced from a hypothetical soluble substrate whose initial concentration would correspond to the concentration of total sugar produced ultimately. Results allowed determining the main parameters in the model, as described below. The apparent Michaelis-Menten constant KM was found to be 217.49 g/L. The apparent rate constant k, which represents the frequency of binding between cellulose and cellulose, was 0.735 h-1. The apparent competitive inhibition constant between total sugar and cellulose was KI is 32.64 g/L. Other model parameters were also evaluated.