BIOSPECKLE AS A NEW TOOL TO DISCRIMINATE BETWEEN FUNGI AND BACTERIA

MARCELO GUZMÁN; MELINA NISENBAUM; HERNÁN SENDRA; ISABEL PASSONI; FROILÁN GONZALEZ; RICARDO ARIZAGA; HECTOR RABAL; SILVIA MURIALDO; MARCELO TRIVI

San Miguel de Tucumán

Congreso; VII CONGRESO ARGENTINO DE MICROBIOLOGÍA GENERAL SAMIGE DEL BICENTENARIO; 2011

Sociedad argentina de microbiología general

Chemotaxis is the movement of microorganisms towards or away chemical gradients. Thisphenomenon has a relevant importance in bioremediation, plant physiology, veterinary,biotechnology and other disciplines.In this work, we present a further development of dynamic speckle methods for the study ofbiological activity in bacteria under swarming assays. We attempt to determine biological activitydifferences between bacteria and other microorganisms. Although there are different opticalmethods to detect bacteria accumulations, none of them can differentiate between degrees ofmotility in the clusters of one image.Experiments using the bacteria Pseudomonas aeruginosa, Pseudmonas putida F1 CheA andthe fungi Phanerochaete chrysosporium RP-78, have demonstrated that the biospeckle candifferentiate fungi from bacteria, and different sort of bacteria in one in situ surface screening.This was achieved for the biospeckle discrimination of different degrees of motility of bacteriaand between the movement of bacteria and fungi. The biospeckle method offers the advantageof being fast, non invasive, inexpensive and accurate.