INSTITUTO DE FISICA DE LIQUIDOS Y SISTEMAS BIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Fractal cell volume regulation
ALVAREZ HUGO ARIEL; CARLEVARO MANUEL; SCHWARZBAUM PABLO JULIO; CHARA OSVALDO
Conferencia; Latin American Conference on Mathematical Modelling of Biological Systems; 2015
Sociedad Argentina de Biofisica (SAB)
Cellular volume is tightly regulated in the animal kingdom: cellexposure to a hypotonic or hypertonic media induces an osmotic cell change followed by a cell volume regulatory response, known as Regulatory Volume Decrease (RVD) or Regulatory Volume Increase (RVI) process, respectively. It has been proposed that this regulatory response would be based on the existence of a mechanical sensor by which the cell would continuously monitor its volume. It has been conceived that this mechanical sensor could be associated with membrane-located mechano-sensitive ion channels, cytosolic molecular crowding or even the cytosqueleton. We developed a mathematical model assuming a mechanical sensor of fractaldimension which contemplates these mechanisms. The model is composed by a system of ordinary differential equations (ODEs) describing the dynamics of cell volume and intracellular solute content and constituting a generalization of previous models1. By constraining the model with experimental information of typical conditions of RVD and RVI assays, the model is able to qualitatively and quantitatively reproduce the phenomenology of the regulatory response. Furthermore, the model predicts a not yet observed oscillatory behavior which can be explained by stability analysis. Chara et al., 2011. J Exp Zool A Ecol Genet Physiol. 315(4): 175-202.Acknowledgment: This work was funded by UBACyT 20020130100027BA (2014-2017) and PIP-CONICET. 112 20110100639 (2012-2014).