INSTITUTO DE FISICA DE LIQUIDOS Y SISTEMAS BIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Proliferation zone in axolotl spinal cord during regeneration.
ROST FABIAN; RODRIGO-ALBORS AIDA; CHARA OSVALDO; DEUTSCH ANDREAS; BRUSCH LUTZ; TANAKA ELLY M
Workshop; EMBO workshop Development and regeneration of the spinal cord.; 2014
We use axolotl spinal cord regeneration after amputation as a model for the regeneration of regenerative mechanisms of the central nervous system of vertebrates.We quantified the number of ependymal cells, their growth fraction and their mitotic events along the anteroposterior axis. We did so for a region from 1-2 mm anterior to the amputation plane up to the tip up until 8 days post amputation (dpa). We analyzed this data with an empirical mathematical model of 2 two spatial zones of cell proliferation along the anteroposterior axis. We fitted parameters using Bayesian inference. This analysis indicates a region close to the amputation plane that shows an increase in cell proliferation, but only from 4 dpa. Interestingly, we could not identify a convergence and extension like mechanism for the extension of the tube during the regeneration in our data.To quantify the cell cycle length we used cumulative BrdU labeling, a standard method to measure the cell cycle lengths and the S Phase duration in anatomically defined populations. We adapted the standard method to the context of spinal cord regeneration by including symmetric cell divisions, a growing population of proliferating cells and quiescent cells to the underlying mathematical model. In agreement with mitotic index data the analysis of the BrdU experiments shows a 3-fold increase in proliferation rate during regeneration.