INSTITUTO DE FISICA DE LIQUIDOS Y SISTEMAS BIOLOGICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
"In Vitro study of the growth and interfacial behavior of Vero and HeLa cells cultures"
EZEQUIEL V ALBANO; HUERGO, ANA MARÍA; B. MOGLIA; GUISONI, N.
Workshop; YITP Workshop: Interface fluctuations and the Kardar-Parisi-Zhang (KPZ) universality class; 2014
Yukawa Institute of Theoretical Physics, KYOTO.
The growth of linear and radial cell colonies is investigated by performing In Vitro cultures.The interfacial properties of the colonies are rationalized in terms of the dynamics scaling theoryand the analysis of the fractal behaviour. Two different cell lines where studied: Verlo and HeLacells. Experiments show that the front of Vero cell colonies moves forward with an averageconstant velocity of v = 0.22 ± 0.02 mm/min, while for HeLa cell colonies such a velocity isslower, namely v = 0.9 ± 0.05 mm/min. The dynamic scaling analysis of both, cultured Veroand HeLa cell colonies, shows that log-log plots of the width of the interface (w) versus thetime (t) collapse in a single curve with a growth exponent = 0.33 ± 0.02. From the analysisof the structure factor a global roughness exponent = 0.50 ± 0.05 is obtained, so that thedynamic exponent is z = 1.5 ± 0.2. On the other hand, the analysis of numerical simulationsof a minimal discrete model for cell cultures in-vitro, aimed to describe the experimental setup,yields = 0.32±0.02, = 0.49±0.05, and z = 1.49±0.2. Summing up, we conclude that thereis an excellent agreement between the experimental and the numerical results. Furthermore,the set of evaluated exponents fulfils the Family-Vicsek relationship and it is consistent with thepredictions of the one-dimensional continuous Kardar-Parisi-Zhang (KPZ) equation ( = 1/3, = 1/2 , and z = 3/2), so that the growing interface of the studied systems can safely beenplaced within the KPZ universality class.