IFLYSIB   05383
INSTITUTO DE FISICA DE LIQUIDOS Y SISTEMAS BIOLOGICOS
Unidad Ejecutora - UE
artículos
Título:
Neutron macromolecular crystallography with LADI-III
Autor/es:
BLAKELEY, MATTHEW P.; TEIXEIRA, SUSANA ; PETIT-HAERTLEIN, ISABELLE; HAZEMANN, ISABELLE; HAERTLEIN, MICHAEL; HOWARD, EDUARDO I; PODJARNY, ALBERTO
Revista:
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2010 vol. 66 p. 1198 - 1205
ISSN:
0907-4449
Resumen:
At the Institut Laue-Langevin, a new neutron Laue diffractometer
LADI-III has been fully operational since March 2007. LADI-III is
dedicated to neutron macromolecular crystallography at medium to high
resolution (2.5-1.5 Å) and is used to study key H atoms and water
structure in macromolecular structures. An improved detector design and
readout system has been incorporated so that a miniaturized reading head
located inside the drum scans the image plate. From comparisons of
neutron detection efficiency (DQE) with the original LADI-I instrument,
the internal transfer of the image plates and readout system provides an
approximately threefold gain in neutron detection. The improved
performance of LADI-III, coupled with the use of perdeuterated
biological samples, now allows the study of biological systems with
crystal volumes of 0.1-0.2 mm3, as
illustrated here by the recent studies of type III antifreeze protein
(AFP; 7 kDa). As the major bottleneck for neutron macromolecular studies
has been the large crystal volumes required, these recent developments
have led to an expansion of the field, extending the size and the
complexity of the systems that can be studied and reducing the
data-collection times required.