Microbial community evolution during restoration of stream Morales (Matanza-Riachuelo basin, Marcos Paz Municipality)

DEL PANNO M.T; MARCONI, P.L.; AGNELLO, A.C.; TRENTINI A.G.; DE CABO, L

Montevideo

Simposio; Microbial community evolution during restoration of stream Morales (Matanza-Riachuelo basin, Marcos Paz Municipality).; 2019

Microbial comMunity evolution DURING restOration OFSTREAM mORALES (Matanza-Riachuelo BASIN, mARCOS pAZ MUNICIPALITY)AgnelloA.C.1, Del Panno M.T.1, de Cabo L.2, TrentiniA.G3, y Marconi P.L.31-CINDEFI, CONICET-UNLP, La Plata,Argentina 2- MACN, CONICET, CABA, Argentina, 3- U. Maimonides-CEBBAD, CONICET,CABA, ArgentinaStream Morales has near its coast, in Marcos Paz Municipality,a landfill (7 ha) that has been used as dumping ground during, almost, 30 years.During 2017, the waste disposal was organized and the landfill was closed.  In order to restore the landfill, the wasteswere removed and the landfill was covered with crushed stone and, afterwards(March 2017-March 2018) March. The restoration processes was ended in December2018 with the assembly of a biocorridor of native flora in theMatanza-Riachuelo basin. Trees, shrubs and herbaceous plants were distributedin 3 ha according to their environmental requirements. Four months later, the vegetalground cover reached up to 92% (initially was only 3% plant cover). Theecosystem services were null at the beginning of the restoration processes andascend to 70 four months later (scale 0 to 100). The objective of the presentwork was to study the diversity of microbial communities developed in the rhizosphereof different plant species used to restore the ex-landfill. Soil samples wereobtained from: 1) a control area without a story of dumping activities, 2) a controlarea in the dumping site that was not restored with vegetation  and 3) the rhizosphere of vegetated restoredarea at the beginning of the intervention process and four months later.  Microbial diversity of soil and rhizospheresamples was studied by DNA extraction, PCR amplification of 16S rRNAhypervariable regions and separation of the PCR products by denaturing gradientgel electrophoresis (DGGE). Clustering analysis using the UPGMA algorithm and the Jaccard coefficient revealed a shift in microbial communities of the rhizosphere of restoredareas over time.  After four months bacterialcommunities of vegetated areas became more similar to the microbiome ofthe control soil that was never used as landfill. The results of thisfirst monitoring indicate that the development of a plant cover could be anauspicious initial step to restore bacterial communities of theex-landfill.