An efficient barley and wheat colonization is achieved by Paraburkholderia tropica after seed inoculation

BERNABEU, PAMELA; LUNA, MARIA FLAVIA; VIO, SANTIAGO; GARCIA, SABRINA SOLEDAD; VIO, SANTIAGO; GALAR, MARIA LINA; GARCIA, SABRINA SOLEDAD; BERNABEU, PAMELA; GALAR, MARIA LINA; LUNA, MARIA FLAVIA

Foz do Iguacu

Simposio; XVI Symposium on Biological nitrogen fixation with non-legumes; 2018

EMBRAPA

Root colonization is a key factor in the successful interaction of Plant Growth Promoting Bacteria with plants. The study of bacterial population dynamic in plant tissues is a direct measure of their efficiency as colonizers. The aim of this study was to determine the colonization patterns of the PGPB Paraburkholderia tropica MTo-293 after its application to barley and wheat seeds to evaluate the efficiency of root colonization under gnotobiotic conditions using different approaches: i-culture-dependent techniques by counting bacterial colonies in suspension of disinfected and non-disinfected plant tissues and ii-culture-independent techniques by microscopic observations of plant tissues inoculated with marked strains with gfp and gus reporter genes, and by molecular biology techniques.