DEVELOPMENT OF 3D-BIOPRINTED PECTIN-BIOCELLULOSE SCAFFOLDS for drug delivery

SERGIO FABIÁN KATZ; HERNANE BARUD; VERÓNICA E. PASSAMAI; GUILLERMO R. CASTRO; VERA A. ALVAREZ

Araraquara - São Paulo

Encuentro; 1º Encontro Brasileiro de Biocelulose; 2018

UNIARA (Universidad de Araraquara)

Introduction: Diabetic foot ulceration represents a major medical, social and economic problem around the world. About 1 in 10 of all ulcers result in amputations and serious complications. Therefore, innovate in treatments that accelerate the chronic wounds healing is imperative. The aim of the project is to develop personalized therapies using 3D-bioprinted dressing scaffolds for the treatment of ulcerated diabetic foot.3D Bioprinter:The bioprinter developed in the Nanobiomaterials Laboratory (NBM-CINDEFI) is a 3D-cartesian printer commanded by an open source Arduino hardware and Marlin firmware. The 3D-bioprinter head was designed to extrude viscous materials using a syringe able to deposit 400 μm-strips over the plate with an air gap among filament of 750 μm.Bioinks: Pectin and Biocellulose exhibit a wide range of functional and biological properties and enjoy a very good public image as natural products for wound healing and tissue engineering [3-5]. Hybrid pectins-cellulose biogels with different properties and compositions were assayed to be injected and making stable gel scaffolds on the printer plate. Bioinks containing 20-25% (w/v) pectin plus 3-5% (w/v) cellulose showed good properties for materialextrusion, being possible to obtain varied scaffold formats.