HYBRID BACTERIAL CELLULOSE - PECTIN FILMS FOR TRANSDERMAL DELIVERY OF BIOACTIVE MOLECULES

DRACHEMBERG F; BOLZAN AD; CACICEDO ML; ALVAREZ VA; CASTRO GR; ISLAN GA; BARTEL LC

Araraquara

Simposio; II International Symposium of Medicinal Chemistry and Regenerative Medicine; 2017

Universidad de Araraquara

Introduction and objectives: Novel biopolymeric films based on bacterial cellulose (BC)modified with high methoxylated pectin (HMP) were developed for transdermal drug delivery.Materials and Methods: The ability of films to incorporate an antibiotic, levofloxacin (Levo),was analyzed. Incorporation efficiencies (EE) were determined using films with differentproportions of HMP (from 0.1% to 2.0%) with a maximum drug payload of 6.23 mg/g. Results:Characterization studies revealed the existence of a cooperative network between both polymersand deep structural changes in BC matrix. Besides, HMP presence decreased water loss in the BCfilm from 93% to 75% after 90 min. Additionally, film incorporation capacity of macromolecules,using Human Serum Albumin (HSA) as a model protein, was studied. HMP presence enhancedin more than 3.5 times the EE of HSA and no pH dependence was observed. Release kineticsof both molecules showed hyperbolic profiles with sustained release. On independentexperiments, HMP presence generated around 50% decrease on both macromolecules releaserates. Additionally, the incorporation of HSA into BC-HMP matrix exhibitied a modulation onLevo release profile. The antimicrobial activity of Levo released from the BC-HMP-HSA filmswas confirmed using Staphylococcus aureus. In-vitro studies revealed no apparent cytotoxicityof the released compounds in mammalian CHO cells. Conclusion: As a conclusion, on thiswork the versatility of bacterial cellulose material was tested by in situ modification with anadditive biopoolymer. The hybrid material exhibited proper characteristic for its application as atransdermal graft with antibiotic properties.