BIOCOMPOSITES BASED ON TPP CROSSLINKED CHITOSAN / BACTERIAL CELLULOSE AS A POTENTIAL STRATEGYFOR CIPROFLOXACIN RELEASE

CACICEDO ML; CASTRO GR; ISLAN GA; BARUD HS; PACHECO G; MENEGUIN AB

Araraquara

Simposio; II International Symposium of Medicinal Chemistry and Regenerative Medicine; 2017

Universidad de Araraquara

Introduction and Objectives: Bacterial cellulose (BC) presents high crystallinity, fibersof nanometric size gives it a greater water hold capacity and not contain lignin, pectin andhemicellulose in its structure. The polymer has been studied, produced and applied in severalareas. Chitosan is a polysaccharide obtained from the N-deacetylation of chitin, consistingof polymeric (1→4)-linked 2-amino-2-deoxy-β-D-glucopyranose units. Because of thebiocompatibility, non-toxicity, biodegradability, and intrinsic antibacterial properties, chitosanis considered as a versatile material with potential biomedical applications. Therefore, the aimof this work was to use bacterial cellulose crosslinked with sodium tripolyphosphate (TPP)and chitosan loaded with ciprofloxacin and to evaluate the antimicrobial capacity and the invitro release study of ciprofloxacin. Materials and Methods: The commercial kitLIVE / DEADBacLight® were used for microbiological assays and the bacteria Pseudomonas aeruginosa andStaphylococcus. aureus were incubated at 24 hours to allow biofilm formation. Subsequently,biofilms were completely covered with empty and ciprofloxacin loaded BC/Chitosan films for 10,30 and 60 min. Controls with untreated bacteria (Live) and HClO treated biofilm (Dead) wereperformed. Then, were observed in a Leica DM 2500 epifluorescence microscope (Germany)equipped with UV filters (495?505 nm) at 400X to determine the viability of the bacteria.Ciprofloxacin release was evaluated in phosphate buffer (10.0mM pH 5,8). Briefly, one film(10 mm) was incubated in 20 mL buffer at 37°C. Samples were taken at different times, andciprofloxacin was measured at the maximum absorbance wavelength (277 nm). Results: For bothPseudomonas aeruginosa and Staphylococcus. aureus, a reduction in the bacterial population wasobserved after 30 and 60 minutes of contact with the bacteria, increasing as time passed. Therelease profile of ciprofloxacin showed a gradual release in 15 min (37%) and 25 min (52%) untila burst in 50 min (80%) and follow constant. After this quickly release, significant percentagesof the amounts of drug released up to 300 min were not observed, suggesting a prolongation ofthe release, which could be exploited for pathologies in which an initial loading dose is required,followed by maintenance of the dose of the antibiotic. Conclusions: The rapid release verified bythe study suggests that the system provides an enough drug for its effectiveness, corroboratingwith the antimicrobial activity test.