VIBRATIONAL MICROSPECTROSCOPY OF INTACT MICROORGANISMS AND OF MICROBIAL BIOFILMS

PETER LASCH; CECILIA FIGOLI; CHRISTOPH SCHAUDINN; MAREN STÄMMLER; ALEJANDRA BOSCH

Victoria

Conferencia; 9th International Conference on Advanced Vibrational Spectroscopy ICAVS; 2017

ICAVS

Vibrational spectroscopic techniques like infrared (IR) and Raman spectroscopy are emerging generic technologies suitable for rapid and reliable characterization of pathogenic microorganisms [1,2]. All these techniques have in common that they can be used to obtain taxon-specific biomarker profiles suitable for bacterial identification at the genus, species, or even at the subspecies level holding the potential to serve as rapid and sensitive identification techniques in clinical or food microbiology and for the detection of highly pathogenic microorganisms. Whilst mass spectrometry applications always require cultivation of the pathogens, vibrational microspectroscopic techniques such as confocal Raman microspectroscopy (CRM), FTIR microspectroscopy and near-field IR nanospectroscopy are principally capable of characterizing individual microbial cells. Vibrational microspectroscopic techniques allow thus for the rapid and sensitive characterization of microorganisms without the need for cultivation, and without the requirement of tagging, labeling or staining.