Synthesis and characterization of solid lipid nanoparticles for controlled release of carbamazepine

SCIOLI MONTOTO S; ISLAN GA; RUIZ MA; CASTRO GR

Trivandrum

Conferencia; International Conference on New Horizons in Biotechnology (NHBT-2015); 2015

CSIR-National Institute for Interdisciplinary Science and Technology Trivandrum, Kerala, INDIA

Solid lipid nanoparticles (SLN) andnanostructured lipid carriers (NLC) have been shown to penetrate the BloodBrain Barrier and allow for greater delivery of drugs to the CNS. They presentthe same advantages of polymeric nanoparticles (NP) but with much lowerintrinsic toxicity. The aim of the present work was the encapsulation of theantiepileptic drug Carbamazepine in SLN and NLC in order to develop acontrolled release delivery system. As lipid phase, cetyl esters, myristyl myristateand Croda®GTCC-LQ-/SG) oil [U1] were used. NP were prepared by ultrasonication of a mixture of themelted lipids and 3% solution of Kolliphor® P188 in water,previously heated at a temperature slightly above the lipids? mp. The drug wasincorporated to the lipid phase, alone or dissolved in 50 ml of DMSO. Formulations were observed by TEM and AFM, showing a mean size range of 50-500 nm. In vitro release studies were performedin 500 ml of KH2PO4 (pH 6.8) using Apparatus 2 USP(paddle) at 100 rpm. Four different formulations were assayed, and all of themshowed high %EE (82.78-93.84%) with released percentages of (29.47-45.30%) and (50.80-78.53%)at 2 and 6 hours, respectively, reaching (70.34-99.59%) at 24 hours.