Studies on PVA Pectin cryogels containing crosslinked enzyme aggregates of keratinase

MARTINAZ, YANINA; CAVELLO, IVANA; CAVALITTO, SEBASTIÁN FERNANDO; ILLANES, ANDRES; CASTRO, GUILLERMO

Lille

Simposio; 10th European Symposium on Biochemical Engineering Sciences and 6th International Forum on Industrial Bioprocesses; 2014

yvinyl alcohol-pectin (PVA-P) films containing enrofloxacin and keratinase were developed to treat wounds and scars produced by burns and skin injuries. However, in order to prevent enzyme inactivation at the interface between the patch and the scars, crosslinked enzyme aggregates (CLEAs) from a crude extract of keratinase produced by Paecilomyces lilacinus (LPSC#876) were synthesized by precipitation with acetone and crosslinking with glutaraldehyde.