“Wheat colonization and plant growth promotion effects by inoculation with Burkholderia tropica”

BERNABEAU P; GARCÍA FERREYRA G.; BOIARDI J L; ZAMORA M; CARRASCO N; LUNA MF

Mar del Plata

Taller; Interacciones Microorganismos-Plantas; 2012

SAMIGE

Green biotechnology is a branch of biotechnology with application in agricultural process mainly to reduce dependence on agrochemical products. the use of plant growth promoting bacteria (PGPB) has gained an important place in the world towards a sustainable agriculture. PGPB are a group of microorganisms able to confer beneficial effects on plant growth and development, without causing damage neither to the host nor the environmental. These microorganisms stimulate plant growth as a consequence of nitrogen fixation, production of phytohormones, enhancement of mineral availability and biocontrol of phytopathogens. PGPB have to colonize and grow on or around the roots for the establishment of an effective plant–microbe interaction. After this necessary step some of them are able to enter roots by different mechanisms and establish endophytic populations. In any case a key feature of plant-beneficial bacteria associations is the efficient colonization of root surfaces. In the present study, have been performed experiments of wheat seeds inoculation to test the ability of Burkholderia tropica to colonize and to evaluate whether it can enhance grain production. Wheat seeds were inoculated with B. tropica Mto293 containing the marker gene gusA in gnotobiotic conditions. On the other hand, wild type strain was used to analyze if colonization was accompanied by stimulation of plant growth under field conditions. Colonization was monitored by plating bacterial suspensions from homogenized tissues and by microscopic localization of bacteria after staining with gus substrate. Plant growth promotion activity was analyzed by measure of fresh weight of roots and aerial parts.