Biodisponibility of ciprofloxacin encapsulated on alginate-pectin gel coacervates

ISLAN GERMAN A; CASTRO GUILLERMO R

Trivandrum

Conferencia; International Conference on New Horizons in Biotechnology; 2011

Ciprofloxacin (Cip) is a wide spectrum and broadly used antibiotic. However, Cip treatment of infections is commonly associated with a poor drug biodisponibility, irritation along the gastrointestinal tract, and several unwanted side effects. In order to overcome such problems, controlled release systems of Cip using gel microspheres composed of biopolymer were analyzed. Screening of Cip with selected biopolymers bring about 90 % antibiotic interaction with a coacervate composed of Alginate/High metoxyl Pectin in 2:1 ratio. Alginate/High Methoxyl Pectin gel microspheres developed by ionic gelation encapsulates 46.8±5.0 % Cip. The gel matrix can release Cip in a sustained manner, releasing 42.7±0.2 % in 2 h under simulated stomach pH conditions, and 83.3±1.1 % Cip release in 80 mM phosphate at pH=7.40 (intestinal). The increase of sodium chloride from 50 to 200 mM implies a Cip release from 69.0±1.5 to 95.1±3.6 % respectively in 2 hour. Scanning electron and optical microscopies analysis demonstrated the cohesive effect of HM Pectin over alginate molecules on the microsphere surface. Those results guarantee all Cip contained in the Alginate/HM Pectin microspheres can be released in a established kinetic profile along the gastrointestinal tract.