COLONIZATION OF DIFFERENT CROPS BY THE PLANT

COUYOUPETROU M; MOYANO D; BERNABEAU P; GALAR M L; TORRES TEJERIZO G; PISTORIO M; LUNA MF

PUERTO MADRYN

Congreso; 46 Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2010

SAIB (SOC. ARGENTINA DE INVESTIGACION BIOQUIM 0327-9545

    MI-P65 COLONIZATION OF DIFFERENT CROPS BY THE PLANT GROWTH PROMOTING-BACTERIUM Burkholderia tropica Couyoupetrou M1, Moyano D1, Bernabeau P1, Galar ML1, Torres Tejerizo G2, Pistorio M2, Luna MF1 1CINDEFI-CONICET. 2IBBM. E-mail: manuelcuyu@yahoo.com.ar The use of biofertilizer and biocontrol organisms is being considered as an alternative or a supplementary way of reducing the use of chemicals in agriculture. These beneficial bacteria are able to colonize roots and, some of them, are also able to colonize plant tissues (endophytes). The understanding of bacterial colonization patterns is a critical prerequisite for the development of effective inoculants. In the present study, colonization patterns of the plant growth promoting bacteria Burkholderia tropica has been monitored by dilution plating assays and microscopic localization in crops of Lycopersicon esculentum, Brassica napus and Sorghum bicolor inoculated with this organism. Microscopic localization of B. tropica (labeled with a stable plasmid carrying the gen for green fluorescent protein) showed that seedling inoculation led to extensive root surface colonization in these plants. This is in accordance with the high epiphytic population densities found in all plants tested (~6-7 log CFU/g fresh weight). Root endophytic bacterial enumerations were also similar for the different crops (~5 log CFU/g fresh weight) and aerial tissues were colonized efficiently (~4 log CFU/g fresh weight). This study provides evidence that B. tropica is able to colonize other plants than its original host and also establish stable associations, at least under our experimental conditions. MI-P65 COLONIZATION OF DIFFERENT CROPS BY THE PLANT GROWTH PROMOTING-BACTERIUM Burkholderia tropica Couyoupetrou M1, Moyano D1, Bernabeau P1, Galar ML1, Torres Tejerizo G2, Pistorio M2, Luna MF1 1CINDEFI-CONICET. 2IBBM. E-mail: manuelcuyu@yahoo.com.ar The use of biofertilizer and biocontrol organisms is being considered as an alternative or a supplementary way of reducing the use of chemicals in agriculture. These beneficial bacteria are able to colonize roots and, some of them, are also able to colonize plant tissues (endophytes). The understanding of bacterial colonization patterns is a critical prerequisite for the development of effective inoculants. In the present study, colonization patterns of the plant growth promoting bacteria Burkholderia tropica has been monitored by dilution plating assays and microscopic localization in crops of Lycopersicon esculentum, Brassica napus and Sorghum bicolor inoculated with this organism. Microscopic localization of B. tropica (labeled with a stable plasmid carrying the gen for green fluorescent protein) showed that seedling inoculation led to extensive root surface colonization in these plants. This is in accordance with the high epiphytic population densities found in all plants tested (~6-7 log CFU/g fresh weight). Root endophytic bacterial enumerations were also similar for the different crops (~5 log CFU/g fresh weight) and aerial tissues were colonized efficiently (~4 log CFU/g fresh weight). This study provides evidence that B. tropica is able to colonize other plants than its original host and also establish stable associations, at least under our experimental conditions.