CINDEFI   05381
CENTRO DE INVESTIGACION Y DESARROLLO EN FERMENTACIONES INDUSTRIALES
Unidad Ejecutora - UE
capítulos de libros
Título:
Study on the effects of organic solvent stress on Bacillus licheniformis S-86
Autor/es:
TORRES SEBASTIAN,; LICIA M, PERA; PANDEY ASHOK; CASTRO, GUILLERMO R
Libro:
Advances in fermentation technology
Editorial:
Asiatech
Referencias:
Lugar: New Dehli; Año: 2009; p. 1 - 13
Resumen:
Introduction There is increasing interest in organic solvent-tolerant microorganisms and their enzymes, which generally display high stability in the presence of non-aqueous solvents (Ogino et al., 1999). Despite the toxicity of the solvent for all forms of life, some organic solvent-tolerant bacteria  capable of thriving in the presence of these toxic compounds have been reported (Isken & de Bont, 1996; Wang et al., 2008). Most of the solvent-tolerant microorganisms reported have been Gram-negative bacteria, which display a cascade of adaptive mechanisms used to acclimatize in the presence of toxic organic solvents. The primary site of organic solvents action is the cell membrane (Sardessai & Bhosle, 2004). Two major mechanisms have been described in these microorganisms. The first one involves the alterations to the cell membrane composition in order to decrease the solvent permeability (Pinkart et al., 1996; Aono & Kobayashi, 1997; Ramos et al., 1997; Tsubata et al., 1997; Heipieper et al., 2003). The second type of adaptive response reduces the accumulation of organic solvents in the inner membrane by transporting the solvent molecules out of the lipid bilayer using active efflux pumps (Isken & de Bont, 1996). Likewise, solvent utilization at high rates or solvent biotransformation to a less toxic product has been observed in some tolerant bacteria (Vangnai et al., 2002; Na et al., 2005). In addition, the changes in overall in Gram-negative microorganism cell morphology in response to organic solvents and other stressful environments have been found (Shi & Xia, 2003; Neumann et al., 2005). On the contrary, only little is known on the effects of organic solvents in Gram-positive bacteria. Gram-positive bacteria lack the outer membrane present in Gram-negative bacteria, and consequently display different mechanisms for surviving with toxic substances in their environment. Thus ...