Comparative study of analytical techniques for deoxynivalenol detection and quantification in wheat samples destined for the manufacture of commercial products

ANDREA ASTORECA; LEANDRO ORTEGA; CECILIA BEATRIZ FÍGOLI; M. CARDÓS; L. CAVAGLIERI; ALEJANDRA BOSCH; TERESA ALCONADA; ANDREA ASTORECA; LEANDRO ORTEGA; CECILIA BEATRIZ FÍGOLI; M. CARDÓS; L. CAVAGLIERI; ALEJANDRA BOSCH; TERESA ALCONADA

World Mycotoxin Journal

World Mycotoxin Journal

Año: 2017 p. 111 - 120

1875-0710

The significant concern regarding toxicity from the presence of deoxynivalenol (DON) in wheat that affects both economy and public health leads to the need to find an appropriate detection method for determining the degree of DON contamination in terms of the equipment available and the speed required for obtaining the incidence. The objective of this study was to compare the performance of two alternative analytical techniques for DON quantification to be used in the food industry with a reference technique. Samples of wheat and the commercial by-products were analyzed by high-performance liquid chromatography (HPLC) with an ultraviolet detector as the reference method and the results compared with those obtained from a rapid lateral-flow immunochromatographic device (Reveal Q+) and the technique of Fourier-transform-infrared (FTIR) spectroscopy. Pearson´s correlation coefficient between the HPLC and Reveal-Q+ data (0.45), although significant (p