Fructans production by Gluconacetobacter diazotrophicus PAL3 in batch cultures.

LUNA MARÍA F.; APREA JULIETA; MOLINARI MARÍA L.; BOIARDI JOSÉ L.

AMIDIQ

Año: 2007

The metabolism of sucrose and the formation of exopolysaccharide (EPS) by Gluconacetobacter diazotrophicus PAL3, in pH-controlled cultures, with sucrose concentrations of 20 and 100 g.l-1 were studied. Levans concentration in culture effluents increased by increasing the sucrose concentration in the medium with about 15% w/w of sucrose conversion in EPS. FOS were also produced either with 20 or 100 g.l-1 sucrose in the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. increased by increasing the sucrose concentration in the medium with about 15% w/w of sucrose conversion in EPS. FOS were also produced either with 20 or 100 g.l-1 sucrose in the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. increased by increasing the sucrose concentration in the medium with about 15% w/w of sucrose conversion in EPS. FOS were also produced either with 20 or 100 g.l-1 sucrose in the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. concentrations of 20 and 100 g.l-1 were studied. Levans concentration in culture effluents increased by increasing the sucrose concentration in the medium with about 15% w/w of sucrose conversion in EPS. FOS were also produced either with 20 or 100 g.l-1 sucrose in the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. increased by increasing the sucrose concentration in the medium with about 15% w/w of sucrose conversion in EPS. FOS were also produced either with 20 or 100 g.l-1 sucrose in the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. increased by increasing the sucrose concentration in the medium with about 15% w/w of sucrose conversion in EPS. FOS were also produced either with 20 or 100 g.l-1 sucrose in the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. concentrations of 20 and 100 g.l-1 were studied. Levans concentration in culture effluents increased by increasing the sucrose concentration in the medium with about 15% w/w of sucrose conversion in EPS. FOS were also produced either with 20 or 100 g.l-1 sucrose in the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. increased by increasing the sucrose concentration in the medium with about 15% w/w of sucrose conversion in EPS. FOS were also produced either with 20 or 100 g.l-1 sucrose in the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. increased by increasing the sucrose concentration in the medium with about 15% w/w of sucrose conversion in EPS. FOS were also produced either with 20 or 100 g.l-1 sucrose in the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. PAL3, in pH-controlled cultures, with sucrose concentrations of 20 and 100 g.l-1 were studied. Levans concentration in culture effluents increased by increasing the sucrose concentration in the medium with about 15% w/w of sucrose conversion in EPS. FOS were also produced either with 20 or 100 g.l-1 sucrose in the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. increased by increasing the sucrose concentration in the medium with about 15% w/w of sucrose conversion in EPS. FOS were also produced either with 20 or 100 g.l-1 sucrose in the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. increased by increasing the sucrose concentration in the medium with about 15% w/w of sucrose conversion in EPS. FOS were also produced either with 20 or 100 g.l-1 sucrose in the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. -1 were studied. Levans concentration in culture effluents increased by increasing the sucrose concentration in the medium with about 15% w/w of sucrose conversion in EPS. FOS were also produced either with 20 or 100 g.l-1 sucrose in the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. -1 sucrose in the medium. In cultures performed with 20 g.l-1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration. -1 sucrose FOS concentration increased early in supernatants but later FOS were consumed. Meanwhile FOS were accumulated all along cultures with 100 g.l-1 sucrose concentration.-1 sucrose concentration.