Immobilized keratinase and enrofloxacin loaded on pectin PVA cryogel patches for antimicrobial treatment

MARTÍNEZ, Y.N.; CAVELLO, I.A.; HOURS, R.A.; CAVALITTO, S.F.; CASTRO, G.R.

BIORESOURCE TECHNOLOGY

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2013 vol. 145 p. 280 - 284

0960-8524

A keratinase isolated from Paecilomyces lilacinus (LPS # 876) was tested against proteins present in the skin but the high enzyme activity was detected on collagen. Keratinase was physically immobilized onto PVA?pectin cryogels and enzyme release was 20.8 ± 2.1%, 63.8 ± 0.2%, 41.5 ± 3.5% and 26.0 ± 3.5% in cryogels containing pectins with esterification degrees (DE) 33.0%, 55.0%, 62.7% and 71.7% respectively at 37 ºC after 3 h incubation. In presence of 0.75 M NaCl, the percentage of enzyme release changed to: 57.5 ± 1.5, 65.8 ± 3.8, 57.3 ± 0.2 and 34.0 ± 4.0 for the four pectins respectively. In-vitro studies of enrofloxacin release from PVA?pectin cryogels at pH close to the human skin (pH = 5.5) showed 15.0% free antibiotic following first order kinetic at 37 ºC after 5 h incubation. However, in the presence of keratinase only 6.9% of enrofloxacin was released under the same experimental conditions.