THE ANTICOAGULANT ACENOCOUMAROL MODIFIES IMMUNOMODULATORY PROPERTIES OF BIFIDOBACTERIA

ASSAD, SABRINA; MINNAARD, JESSICA; PEREZ, PABLO F

Congreso; LVI SAIB Meeting ?XV SAMIGE Meeting; 2020

The microecology of the intestinal tract involves complex interaction between the microbiome, nutrients and drugs. Maintaining intestinal homeostasis is important since disturbing this balance could lead to an uncontrolled inflammatory response. Considering that probiotic microorganisms present in functional foods can coexist with medications we proposed to study the effect of acenocoumarol (AC) (an anticoagulant used for the treatment of thromboembolic disorders) on the polarization of THP-1 cells induced by bifidobacteria. Previously we demonstrated, in THP1 cells, that AC partially abrogates phagocytosis of bifidobacterial as well as the increase of HLA-Dr and TLR2 expression induced by these bacteria. The monocytic cell line THP-1 was used undifferentiated and differentiated to macrophages with phorbol 12-myristate 13-acetate (PMA). Cells were stimulated in DMEM for 18 h at 37°C, 5% CO2 with AC (0.016 mg ml-1), Bifidobacterium bifidum CIDCA 5310 or B. adolescentis CIDCA 5317 (multiplicity of infection=10 bacteria/cell), and INF-γ (375 ng/ml) + LPS (0,5 µg/ml) or IL-4 (10 ng/ml) as positive controls for expression of CD16 and CD64 or CD163, CD23 and CD206, respectively. The expression index (EI) was calculated as the percentage of positive cells x mean fluorescence intensity after flow cytometry analysis. Results showed that acenocoumarol significantly (p