CIDCA   05380
CENTRO DE INVESTIGACION Y DESARROLLO EN CRIOTECNOLOGIA DE ALIMENTOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Production of reactive oxygen species by intestinal epithelial cells triggered by interaction with probiotic microorganisms
Autor/es:
ROMANIN D E; GONZáLEZ MACIEL D; HIRIART Y; GARROTE G L; RUMBO M
Lugar:
Buenos Aires. Argentina
Reunión:
Congreso; 1º Congreso Franco Argentino de Inmunología, LVIII Reunión Anual de la Sociedad Argentina de Inmunología; 2010
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
Different probiotic microorganisms may modulate intestinal immune response, however the mechanisms involved remain poorly characterized. Modulation of intestinal epithelial response may contribute to probiotic function. Different intracellular signaling pathways have been shown to be modulated by epithelial-microorganism interaction. Kumar et. al 1,2 described that butyrate or certain strains of lactobacilli induce endgenous production of Reactive Oxygen Species (ROS) on intestinal epithelial cells. This lead to a inhibition of Neddylation of Cullin 1, a required step for activation of ubiquitin ligase, thus inhibiting ubiquitination and posterior proteasomal degradation of I-kB. This may lead to an anti-inflammatory effect of ROS induction. We have previously described that Kefir-isolated yeast have a marked anti-inflamatory capacity and that in kefir-isolated bacteria this capacity was lower Differential targeting to the proteasome of key players of inflammatory pathways such as IkB-á protein can be modulated by probiotic bacteria by a mechanism dependent on the production of ROS. The aim of the present work was to set up a system to study this phenomena on intestinal epithelial cells and test the effect on this pathway by a panel of probiotic bacteria and yeast.