REGULATORY T CELLS INDUCED BY ACTINOMYCES BACTERIA CONTROLLED THE ALLERGIC REACTION IN A MOUSE MODEL OF FOOD ALLERGY

SMALDINI PAOLA; TREJO FERNANDO M; MAHAVIR SINGH; DOCENA, GUILLERMO H

Congreso; I meeting LASID-FAIC-SAI; 2015

Food allergy is a rising health concern worldwide and it is accepted that it is caused by a breakdown in immunologic tolerance. The most efficient treatment consists of the strict avoidance of food allergens, although sometimes it is a hard and risky task. Defects in Treg cells have been reported in patients with food allergies, suggesting that an impaired tolerance may play a key role in the intestinal allergic inflammation. We aimed to modulate milk allergy in a mouse model through the oral administration of a heat-killed Actinomyces bacteria (Tsukamurella inchonensis-Ti). Mouse model: a cholera toxin -driven Th2 specific immune response was promoted in Balb/c mice by gavage, and hypersensitivity reactions were evidenced immediately after the oral challenge with CMP.In vivo parameters: clinical score and skin test were analyzed.In vitro parameters: serum specific isotypes, cytokines, mucosal Tregs and cytokines, were assessed.Therapeutic strategies: Ti (108 bacteria) was orally administrated during two months. Ti induced regulatory T cells that controlled the Th2-mediated allergic responses. IL-10 production may have a critical role controlling inflammation. Treg depletion abolished the modulation of the immune responses.