CIDCA   05380
CENTRO DE INVESTIGACION Y DESARROLLO EN CRIOTECNOLOGIA DE ALIMENTOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Evaluation of prolonged exposure to microcystin in mice
Autor/es:
GIANNUZZI L., SEDAN D., LAGUENS M., COPPARONI G., ARANDA O., MARRA C. A. AND ANDRINOLO, D.
Lugar:
Florencia
Reunión:
Congreso; The International Association of Forensic Toxicologists, TIAFT, Florencia, Italia, setiembre, 2015.; 2015
Institución organizadora:
TIAFT
Resumen:
Introduction: Cyanobacterial blooms in natural waters, causing serious water pollution and public health hazard to humans and livestock, have become a worldwide problem. Species of the genus Microcystis, are known for their potential ability to synthesize toxins, mainly microcystins (MC). The effects of prolonged exposure to low dosis of MCs on health are not yet sufficiently understood and this type of poisoning is often undiagnosed. Aims: The objective of this work was to explore different tissue damages generated by oral prolonged exposure to 50 to 100 µg MC-LR/kg in a murine model. Methods: MC-LR was purified from natural blooms of M. aeruginosa collected at the Rio de la Plata basin, Argentina. The purification was performed with semi-preparative HPLC HP 1100 with diode array detector system. The toxin was tested by the HPLC?MS method. Thiobarbituric acid reactive substances (TBARS), superoxide dismutase activity (SOD) and glutathione profile were study in different tissue. In addition, the determination of MC-LR levels in liver, kidney, plasma, urine and feces of treated mice was carried out.Results: We found alterations in TBARS, SOD activity and glutathione profile in liver and intestine of mice exposed to both doses of MC-LR tested in this work. In addition the presence of MC-LR was detected in both organs. Also, we found 3.6 ± 0.6% and 15.3 ± 1.6% of hepatic steatosis and intraepithelial lymphocytes decrease of 28.7 ± 5.0% and 44.2 ± 8.7% in intestine of 50 and 100μg MC-LR/kg treated animals respectively. This result could have important implications in mucosal immunity, since intraepithelial lymphocytes are the principal effectors of this system. Conclusion: Our results indicate that intermittent and prolonged oral exposure at doses as low as 50 μg MC-LR/kg each 48 hs generates damage not only in liver but also in intestine. This finding is interesting taking into account that a dose of 40 μg MC-LR/kg (derived from experiments of daily administration) is a NOAEL actually accepted.