Hepatic and intestine alteration in mice after oral low doses of Microcystin-LR prolonged exposure

SEDAN D., LAGUENS M., COPPARONI G., ARANDA O., GIANNUZZI L., MARRA C.A., ANDRINOLO D.

Lisboa

Congreso; Congreso Ibérico de Cianotoxinas. VI Reunión de la Red Ibérica de Cianotoxinas, Lisboa,; 2015

Red Ibérica de Cianotoxinas

MC-LR oral prolonged exposure is worrying since implicate the principal route of exposure and could lead to liver damages which has not had specific symptomatology. Also this kind of exposure is not enough explored yet. The aim of this work was to explore the liver and intestine damages generated by oral prolonged exposure to 50 and 100 µg MC-LR/kg in a murine model. We found a 10% and 35% of hepatic steatosis for 50 and 100μg MC-LR/kg treated animals respectively and a dose dependent intraepithelial lymphocytes decrease. Also, were registered alterations in TBARS, SOD activity and glutathione profile in liver and intestine of mice exposed to both doses of MC-LR. In addition the presence of MC-LR was detected in both organs which indicate that the damage generated in liver (main target of the toxin) and intestine (the most important organ involved in the toxin absorption) is generated by the contact whit MC-LR. Our results indicate that intermittent oral exposure at doses as low as 50 μg MC-LR/kg generates damage not only in liver but also in intestine.)