CIDCA   05380
CENTRO DE INVESTIGACION Y DESARROLLO EN CRIOTECNOLOGIA DE ALIMENTOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
A natural bacterial inoculum in maternal milk
Autor/es:
PÉREZ, P. F.; DORÉ, J.; LECLERC, M. ; LEVENEZ, F. ; BENYACOUB, J. ; SERRANT, P. ; SEGURA-ROGGERO, I. ; SCHIFFRIN, E. J. AND DONNET-HUGHES, A.
Lugar:
Barcelona
Reunión:
Congreso; 40th Annual Meeting of the European Society for Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN); 2007
Resumen:
Aim: To study the presence and origin of a bacterial inoculum in maternal
milk.
Methods: The presence of viable bacteria in the milk and peripheral blood of
healthy mothers was determined by plating. In addition, Temporal
Temperature Gradient-Gel Electrophoresis (TTGE) was used to examine
bacterial ribosomal DNA (rDNA) content in maternal peripheral blood
mononuclear cells, milk cells and faeces as well as in the corresponding
infant faeces. Blood from non-gravid, non-lactating women served as a
control. Bacterial translocation to extraintestinal tissues was also evaluated in
virgin, pregnant and lactating mice.
Results: The total number of viable bacteria in breast milk was <103cfu/mL
but maternal blood and milk cells contained the DNA of diverse enteric
bacteria. Some bacterial DNA signatures were common to infant feces and to
samples of maternal origin. Bacterial translocation to mesenteric lymph
nodes and the mammary gland occurs during late pregnancy and early
lactation in mice.
Summary: Bacterial translocation is increased during pregnancy and
lactation in mice. Human breast milk cells contain a low number of viable
bacteria but a range of bacterial DNA that is also found in maternal blood
cells.
Conclusion: Our results suggest that during lactation, bacterial components
are transported from the intestine to the lactating breast within host cells. The
consequences of this trafficking for the mother and her baby are not known.
We speculate that it educates the neonatal immune system to recognise
specific molecular motifs of bacteria and to respond appropriately to
pathogens and commensals.