CIDCA   05380
CENTRO DE INVESTIGACION Y DESARROLLO EN CRIOTECNOLOGIA DE ALIMENTOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Immunoproteomic studies for epitope mapping of cross-reactive allergens of cow´s milk and soy as an approach to mucosal vaccine design
Autor/es:
TREJO SA, CANDREVA A, FERRER M, BRONSOMS S, DOCENA G, PETRUCCELLI S
Lugar:
Bilbao
Reunión:
Congreso; The 2nd Metabolomics & Proteomics Workshop Technologies and Applications; 2012
Institución organizadora:
CIC bioGUNE
Resumen:
Soy-based formulas are
frequently used as a dairy substitute in paediatric patients with cow´s milk
allergy (CMA). However, clinical intolerance has been observed in some patients
and it might be due to the cross-reactivity between these allergenic systems.
Bovine caseins, and 7S and 11S soy glycinins have been described as
cross-reactive allergens and we propose to explore the immunomodulation of CMA
through the use of cross-reactive components by immunotherapy.
Novel therapeutic strategies
beyond the strict avoidance of allergen exposure are urgently needed and immunotherapy
is an attractive optionfor specific and inexpensive corrective treatment for
many diseases. A key point for this therapeutic approach isthe identification
of the antigenic component that can be use to induce an immune component that may
either block the antigen (neutralization), or induce specific regulatory T
cells (tolerance).
Using monoclonal antibodies
(mAb) specific to bovine caseins, we have
investigated the antigenic properties of CM and soy proteins. In order to
identify the epitopes recognized by these mAbs, purified caseins were
enzymatically digested. The resulting peptides were separated by RP-HPLC and
analyzed by dot blot using three casein-specific mAbs, and finally they were
identified by MALDI-TOF MS. In parallel, antibodies were immobilized onto
magnetic beads and afterwards incubated with the peptide mixture previously
obtained by proteolysis. The MALDI-TOF analysis was performed to compare bound
and unbound peptide profiles. Using different proteolytic enzymes we have shown
that casein-specific mAbs recognize at least three peptides (two on αS1-casein
and one on Κ-casein). By means of bioinformatic tools we have found that these
immunogenic epitopes are also expressed in different soy proteins previously described as
allergens.
In conclusion, we have
detected cross-reactive soy and milk epitopes that could be use as vaccine
candidate to develop tolerance in patients with food allergy.