Niveles de hidroperoxidosy metahemoglobina como biomarcadores de efecto de una exposición prolongada a Microcystina-LR

SEDÁN D., ROSSO L., GIANNUZZI L, Y ANDRINOLO D.

La Plata

Congreso; 7 mo Congreso de Medio Ambiente (AUGM); 2012

The effects on health by prolonged exposure to microcystins is not yet sufficient understood and this type of poisoning is often undiagnosed. While chronic exposure has been linked with liver cancer and had been described alterations in marker enzymes of liver damage in exposed populations, has have not a profile of parameters that indicate exposure to microcystins. The aim of this work is to determine, based on an animal model of prolonged exposure by successive i.p. doses of 25 µg MC-LR/kg body weight, several plasma parameters wich could be useful as exposure biomarkers. On blood samples hemoglobin (Hb) and methemoglobin (MetHb) levels were determined. We also studied plasma levels of hydroperoxides (ROOHs), α-tocopherol, glutathione and lipid profile as well as SOD and CAT erythrocyte activities. In addition was carried out the determination of MC-LR levels in liver, kidney, plasma, urine and feces of treated mice. We found that alteration in MetHb, ROS, glutathione, α-tocopherol levels, SOD activity and plasma lipid profile correlates with those expected if the alteration derived from the hepatic damage. The alterated plasma paramenters could be used as effect biomarkers in epidemiological studies.