CONICET | Buscador de Institutos y Recursos Humanos

Lecithins are widely used in the food industry because of their multifunctional characteristics. Fractionation of the original mixture of phospholipids in lecithin is desirable for certain applications. The influence of ethanol:water mixtures (90:10 - 96:4) and other operative conditions (temperature 35-65ºC, incubation time 30-90 min), solvent/lecithin ratio 2:1, 3:1 on the extraction of PC enriched fractions of sunflower lecithin (a non GMO product) were investigated. Yield % and phospholipid composition of the enriched PC fractions as well as the residue were determined. The percent extraction coefficient of each phospholipid (%EPC, %EPE and %EPI) in the enriched PC fraction was calculated. Values of %EPC varied from 6.5 (35°C, 30 min, 2:1, 90:10) to 52.6 (65°C, 90 min, 3:1, 96:4). The high levels of temperature and incubation time produced a significant increase of this coefficient (p <0.05) while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. from 6.5 (35°C, 30 min, 2:1, 90:10) to 52.6 (65°C, 90 min, 3:1, 96:4). The high levels of temperature and incubation time produced a significant increase of this coefficient (p <0.05) while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. from 6.5 (35°C, 30 min, 2:1, 90:10) to 52.6 (65°C, 90 min, 3:1, 96:4). The high levels of temperature and incubation time produced a significant increase of this coefficient (p <0.05) while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. while the high water content in the ethanolic mixture recorded a considerable decrease in phosphatidylcholine extraction. %EPI (<3%) values showed the high insolubility of phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistical analysis and response surface methodology evidenced the influence of the different variables on the extraction of PC enriched fractions at laboratory scale. phosphatidylinositol. Statistica