Simulated gastrointestinal conditions increase adhesion ability of Lactobacillus paracasei strains isolated from kefir to Caco-2 cells and mucin.

CARASI P; SARRADELL M.A; ZAVALA L.; BRONSOMS S.; ABRAHAM A G; BENGOA A.A; TREJO S.A.; GARROTE G L

FOOD RESEARCH INTERNATIONAL

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2017 vol. 103 p. 462 - 467

0963-9969

Gastrointestinal conditions along the digestive tract are the main stress to which probiotics administrated orallyare exposed because they must survive these adverse conditions and arrive alive to the intestine. Adhesion toepithelium has been considered one of the key criteria for the characterization of probiotics because it extendstheir residence time in the intestine and as a consequence, can influence the health of the host by modifying thelocal microbiota or modulating the immune response. Nevertheless, there are very few reports on the adhesionproperties to epithelium and mucus of microorganisms after passing through the gastrointestinal tract. In thepresent work, we evaluate the adhesion ability in vitro of L. paracasei strains isolated from kefir grains after acidand bile stress and we observed that they survive simulated gastrointestinal passage in different levels dependingon the strain. L. paracasei CIDCA 8339, 83120 and 83123 were more resistant than L. paracasei CIDCA 83121 and83124, with a higher susceptibility to simulated gastric conditions. Proteomic analysis of L. paracasei subjectedto acid and bile stress revealed that most of the proteins that were positively regulated correspond to the glycolyticpathway enzymes, with an overall effect of stress on the activation of the energy source. Moreover, it isworth to remark that after gastrointestinal passage, L. paracasei strains have increased their ability to adhere tomucin and epithelial cells in vitro being this factor of relevance for maintenance of the strain in the gut environmentto exert its probiotic action.