CIDCA   05380
CENTRO DE INVESTIGACION Y DESARROLLO EN CRIOTECNOLOGIA DE ALIMENTOS
Unidad Ejecutora - UE
artículos
Título:
Oral administration of kefiran exerts a bifidogenic effect on BALB/c mice intestinal microbiota
Autor/es:
HAMET M F; ABRAHAM A. G; PEREZ P; MEDRANO M.
Revista:
Beneficial Microbes
Editorial:
Wageningen Academic
Referencias:
Año: 2016 vol. 7 p. 237 - 246
ISSN:
1876-2883
Resumen:
ABSTRACTActivity of kefiran, the exopolysaccharide present in kefir grains, was evaluated on intestinalbacterial populations in BALB/c mice. Animals were orally administered with kefiran andEubacteria, lactobacilli and bifidobacteria populations were monitored in faeces of mice atdays 0, 2, 7, 14 and 21. Profiles obtained by Denaturing Gradient Gel Electrophoresis(DGGE) with primers for Eubacteria were compared by principal component analysis andclearly defined clusters, correlating with time of kefiran consumption, were obtained.Furthermore, profile analysis of PCR products amplified with specific oligonucleotides forbifidobacteria showed an increment in the number of DGGE bands in the groups administeredwith kefiran. Fluorescent In Situ Hybridization (FISH) with specific probes for bifidobacteriashowed an increment of this population in faeces, in accordance to DGGE results.Bifidobacteria population was also studied on distal colon content after 0, 2 and 7 days ofkefiran administration. Analysis of PCR products by DGGE with Eubacteria primers showedan increment in the number and intensity of bands with high GC content of mice administeredwith kefiran. Sequencing of DGGE bands confirmed that bifidobacteria were one of bacterialpopulations modified by kefiran administration. DGGE profiles of PCR amplicons obtainedby using Bifidobacterium or Lactobacillus specific primers confirmed that kefiranadministration enhances bifidobacteria, however no changes were observed in Lactobacilluspopulations. The results of the analysis of bifidobacteria populations assessed on differentsampling sites in a murine model support the use of this EPS as a bifidogenic functionalingredient.