CIDCA   05380
CENTRO DE INVESTIGACION Y DESARROLLO EN CRIOTECNOLOGIA DE ALIMENTOS
Unidad Ejecutora - UE
artículos
Título:
Hepatic and intestine alterations in mice after prolonged exposure to low oral doses of Microcystin-LR
Autor/es:
DANIELA SEDAN.; LAGUENS MARTÍN; GUIDO COPPARONI; OSWALD ARANDA; LEDA GIANNUZZI; DARÍO ANDRINOLO.
Revista:
TOXICON
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Lugar: Amsterdam; Año: 2015 vol. 104 p. 23 - 36
ISSN:
0041-0101
Resumen:
Oral intake of Microcystin-LR (MC-LR) is the principal route of exposure to this toxin, with prolonged exposure leading to liver damage of unspecific symptomatology. The aim of the present paper was therefore to investigate the liver and intestine damage generated by prolonged oral exposure to low MC-LR doses (50 and 100 ug MC-LR/kg body weight, administrated every 48 h during a month) in a murine model. We found alterations in TBARS, SOD activity and glutathione content in liver and intestine of mice exposed to both doses of MC-LR. Furthermore, the presence of MC-LR was detected in both organs. We also found hepatic steatosis (3.6± 0.6% and 15.3± 1.6%) and a decrease in intraepithelial lymphocytes (28.7±5.0% and 44.2± 8.7%) in intestine of 50- and 100-ug MC-LR/kg treated animals, respectively. This result could have important implications for mucosal immunity, since intraepithelial lymphocytes are the principal effectors of this system. Our results indicate that prolonged oral exposure at 50mg MC-LR/ kg every 48 h generates significant damage not only in liver but also in intestine. Thisfinding calls for a re-appraisal of the currently accepted NOAEL (No Observed Adverse Effect Level), 40 ug MC-LR/kg body weight, used to derive the guideline value for MC-LR in drinking water. Hepatic and intestine alterations in mice after prolonged exposure to low oral doses of Microcystin-LR.