Microstructure and biological evaluation of zirconia scaffolds coated by fluorapatite

B. LEON; E. PRADP FERRAZ; L. B. GARRIDO; P. TAMBASCO DE OLIVEIRA; M. P. ALBANO; A. L. ROSA

Congreso; 61º Congresso Brasileiro de Ceramica; 2017

Highly porous zirconia (ZrO2) scaffolds fabricated by the replication method were coated with fluorapatite (FA). The FA coating was obtained by dipping the ZrO2 scaffolds into aqueous FA slips having different viscosity values (1.0, 2.2 and 5.0mPa.s). The influence of the FA slip viscosity on the reduction of the scaffold porosity and microstructure of the coated scaffolds were investigated. In addition, cell spreading and survival of bone marrow-derived stromal cells (BMSC) and pre-osteoblastic MC3T3-E1 cells on the uncoated and coated scaffolds were examined. The porous structure obtained after coating with slips of 2.2 mPa.s viscosity for 2 sec., with a low reduction of the porosity and pore size by the FA layer and a continous film distributed along the strut surfaces, enabled adhesion, spreading and survival of BMSC and MC3T3-E1 cells over a 7-day culture period, with no significant differences compared with the uncoated ZrO2 scaffold.