Biocompatibility of core@shell particles: cyto- and genotoxic study of colloidal silica spheres coated with crystalline or amorphous zirconia in human osteosarcoma cells

DI VIRGILIO A.L.; ARNAL P.M; MAISULS, I.

MUTATION RESEARCH. GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2014 vol. 770 p. 85 - 94

1383-5718

Cyto and genotoxic effects of novel spherical particles with an amorphous core of silica and a crystalline or amorphous shell of circonium oxide (SiO2@ZrO2) had been study on a human osteoblast-like cell line (MG-63). Only the one with the crystalline shell reduced cell viability at 100 μg/mL associated to an increase on ROS formation and decrease of GSH/GSSG ratio. Moreover, this material induced DNA damage detected by the Comet and MN assay, from 5 and 25 μg/mL,respectively. However, the amorphous analogue induced only a genotoxic action from 10 and 50 μg/mL according to the Comet and MN assays. According to TEM photographs, both were found inside the cells forming vesicles, however none of them entered the nucleus.